Molecular and biophysical mechanisms behind the enhancement of lung surfactant function during controlled therapeutic hypothermia

Abstract Therapeutic hypothermia (TH) enhances pulmonary surfactant performance in vivo by molecular mechanisms still unknown. Here, the interfacial structure and composition of lung films have been analysed vitro under TH as well basis its improved both physiological inhibitory conditions. The biophysical activity a purified porcine was tested slow breathing-like dynamics constrained drop surfactometry (CDS) captive bubble surfactometer (CBS) at 33 37 °C. Additionally, temperature-dependent also upon inhibition plasma subsequent restoration further supplementation. Interfacial correlated with lateral lipid made native surfactant. Lipid/protein mixtures designed models to mimic different contexts were studied. capability drastically reduce surface tension enhanced Larger DPPC-enriched domains lower percentages less active lipids detected exposed TH-like Surfactant resistance boosted therapies more effective This may explain respiratory outcomes observed cooled patients acute distress syndrome opens new opportunities treatment injury.